Vidya Rajasekaran

3 Questions 5 Answers 0 Followers

Questions related from Vidya Rajasekaran

What happens to the DNA from sloughed off intestine epithelial cells when extracting bacterial DNA from mouse fecal samples?

I am currently using the PowerSoil DNA extraction kit from MoBio for DNA extraction from bacteria present in mouse fecal matter. I wanted to know how the sloughed off intestine epithelial cells...

22 August 2016 5,200 1 View

mRNA to protein expression?

I have been conducting a series of experiments where I transiently transfect plasmid with my gene of interest into HT29 cells. When I performed qRT-PCR on these cell samples, I can clearly see an...

04 December 2013 7,021 8 View

Can protein expression stop in cell lysates?

I collected whole cell lysates of HT29 Clones in RIPA buffer (Protease Inhibitor cocktail, PMSF, Na3VO4 and NaF all added into the RIPA buffer) and analyzed them by western blot on the same day...

20 November 2013 8,677 4 View