Victoria Gadd

3 Questions 3 Answers 0 Followers

Questions related from Victoria Gadd

Has anyone been able to release cells, therefore RNA, from the generic HyStem (hyaluronic acid hydrogel) kit from Sigma?

I have some interesting results from my cells cultured in Sigma's HyStem basic scaffold kit and would like to assess changes in gene expression. I was wondering if anyone who has been using this...

03 March 2017 3,712 1 View

Sirius red staining following single chromogen detection?

I am trying to detect Pan-keratin by immunohistochemistry with a Sirius red counterstain. I have no problems with the Sirius red staining normally, however as soon as my sections have been exposed...

08 August 2013 7,992 8 View

Is it okay to perform FACS runs on the benchtop with PBMCs from patients with HCV?

The cells get fixed halfway through the protocol.

10 October 2012 1,369 2 View