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Hi, I have performed an RT-qPCR for relative quantification. The SOI is frmA and reference gene is topB. I believe there should be no Cq for the control samples labelled as NRT...
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Hi, I am doing a qPCR and using a reference primer for my lab research project. However, I am not sure what is the purpose of having a singular primer for my reference gene and having both...
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