Victoria Cerrada López

2 Questions 7 Answers 0 Followers

Questions related from Victoria Cerrada López

Why can't I obtain homozyogous clones by CRISPR editing?

Hi everybody, I'm trying to perform CRISPR-Cas9 editing on an iPSC line in order to correct a single nucleotide mutation. I'm using ribonucleoprotein complexes (RNPs) to the delivery of the Cas9...

11 January 2022 8,115 10 View

Has anyone reprogrammed PBMCs to iPSCs using matrigel and ReproTeSR?

Hi everybody, I'm trying to reprogram PBMCs isolated from peripheral blood to iPSCs using Cytotune 2.0 kit. I've tried to do it feeder-free using Matrigel for coating and ReproTeSR (StemCell...

30 September 2019 3,115 9 View