Victoria Cairoli

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Questions related from Victoria Cairoli

How much RNA do you obtain from 200 ul plasma with commercial kits?

Hi everyone! I am extracting total RNA enriched in microRNAs with miRNeasy Serum/Plasma kit (QIAGEN) and I am obtaining low concentration yields aprox 15 ng/ul (elution in 14 ul of RNAse-free...

30 May 2023 1,793 4 View

What TRIZOL and cloroform temperature are best for RNA extraction?

I am extracting miRNA-enriched RNA with miRNeasy Serum/Plasma Kit (QIAGEN #217184) and the addition of TRIZOL and cloroform before the centrifugation at 4ºC made me wonder if these reactives have...

18 May 2023 5,582 1 View