Victor Pastre

2 Questions 1 Answers 0 Followers

Questions related from Victor Pastre

Should I include the entire mRNA genetic sequence or just the CDS to express a recombinant protein?

I want to express a recombinant protein, but I am unable to obtain the mRNA sequence encoding this protein. Therefore, I intend to use a sequence available in a database. Thus, I would like to...

14 July 2023 2,011 3 View

Simultaneously expression of two different genes using the same plasmid for each one can impact the expression level?

We are trying to express a recombinant protein that is made of two different subunits. We have a plasmid with the alpha subunit and another with the beta subunit, but they are derived from the...

13 June 2023 3,835 5 View