Victor A. Leon

4 Questions 3 Answers 0 Followers

Questions related from Victor A. Leon

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

08 October 2020 1,027 3 View

Plasmid compatibility with Rosetta 2 (DE3) competent cells?

Hello I have been trying to transform pet28b plasmids into Rosetta cells. I have tried two plasmids that already show expression in BL21 ( DE3) cells. Is there a list of compatible plasmids that...

21 July 2016 5,139 4 View

Any Fluorescence techniques recommended for protein interactions?

Is there any fluorescence technique you would recommend for protein interactions? I work with phosphoproteins. Most are cytoplasmic proteins and some are domains from transmembrane proteins. Is...

15 March 2016 6,916 4 View

Part of membrane protein is found in pellet rather than in the supernatant, any idea why?

I have expressed human protein -part of membrane protein- in bacteria.The protein is found in the pellet rather than in the supernatant.Is a His- tag protein. Any idea why?

13 March 2016 9,451 4 View