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Questions related from Victor Gonzalez Huici
We are trying to detect variants at very low frequency in human tissues using WES (UMI-UDI libraries), aiming for a mean depth of 3000x, but we are getting very high duplication rates. In our...
28 November 2022 3,783 0 View
We performed a CRISPR to knock out XPF in a RPE1 cell line and want to "pre-validate" clones through UV-C hypersensitivity (254 nm lamp). Which dose would you suggest to use to maximize...
18 February 2020 8,125 3 View
Hi all We are doing some scRNAseq from human tissues and getting poor survival (as by TrypanBlue) after FACS sorting, even if we select for DAPI- in FACS. We suspect FACS is stressing/killing...
10 September 2018 1,367 5 View
We want to expand single T cells (CD4+ plus CD8+), non-antigen specific, in 96- or 382-well plates. Any advice will be welcome, in particular on the plate type and how to best stimulate the cells....
01 January 1970 9,946 0 View
