10 Questions 21 Answers 0 Followers
Questions related from Vicken Aknadibossian
Hello, I am trying to do DNA extraction from mites, for molecular analysis should i store the mites in 70% or 96% ethanol? Either way, later on when i want to crush the mites and add CTAB... do...
06 June 2018 2,413 2 View
Hi i'm performing dot blot immunoassay, using nylon membrane Becaus my antibodies are anti a short peptide and not the whole native protein(and not from terminal areas) I'm afraid the native...
05 May 2018 701 5 View
Hi, I tried to express membrane proteins in a cell-free system with the addition of liposomes, when i take a sample of the reaction and mix it with SDS loading buffer and incubate at 37C for 1...
04 April 2018 1,829 8 View
I was going to order short peptides 20-30AA conjugated to KLH to immunize a rabbit for antibodies against a plant pathogen. Fortunately i stumbled across this article which reports KLH-antibodies...
02 February 2018 1,922 4 View
Hello, I am designing to produce antibodies against 15aa peptides, these antibodies will be used for detection of a pathogen in plant. The problem is when i blast these 15aa sequences there are...
02 February 2018 6,063 3 View
Hi, I am planning to express a membrane protein in a cell-free system. My ultimate goal is to use the protein to immunize a rabbit and obtain antibodies. If i do it without nanodiscs , the...
01 January 2018 8,323 7 View
Hey, So i have a 39kDa Membrane Protein, cloned into pRSET C and transformed into C43, sequenced to makes sure everything is there and made a glycerol stock of. From that glycerol stock i start a...
12 December 2017 7,412 3 View
When i run as SDS page gel first of all it takes about 3 hours for the dye to get there on a 110V and secondly this weird as if band (indicated by arrows) is appearing and stopping the separation...
11 November 2017 994 6 View
If i Lyse cell overexpressing a membrane protein with sonication in 20mM NaH2PO4 and 500mM Nacl, firstly can i load it as it is in sds page or do i have to add SDS loading buffer? Either way ,...
11 November 2017 1,568 9 View
So, i am double digesting pRSET A vector and an insert with BamHI and PstI ... running a gel and purifying the respective bands.... After ligation and transformation , and running PCR on the...
10 October 2017 6,497 4 View
