Vicente Planells-Palop

3 Questions 2 Answers 0 Followers

Questions related from Vicente Planells-Palop

How can I avoid the edge effect (antibody/dye binding the edges of mass tissue section) in tissue immunostaining (IHC & IF) ?

I am getting very specific staining using my antibodies; however, I also get (sometimes) positive staining around the edges of my preparation (tissue sections, FFPE). I have tried both for antigen...

01 May 2015 1,262 5 View

How can I get rid of these spots on a gDNA Southern?

This is a standard NaOH Southern transfer using good quality agarose. Unincorporated nucleotides were removed, but I still get spots on my film.

06 February 2015 9,416 2 View

Can I add Pen/strep to my hESC cultures?

Hi, I was just wondering whether I can add antibiotics to avoid contamination into my human embryonic stem cell cultures without interfering with the cells. Any suggestion? concentrations? Thanks...

18 December 2014 5,150 5 View