Vicente Planells-Palop

4 Questions 4 Answers 0 Followers

Questions related from Vicente Planells-Palop

How can I avoid the edge effect (antibody/dye binding the edges of mass tissue section) in tissue immunostaining (IHC & IF) ?

I am getting very specific staining using my antibodies; however, I also get (sometimes) positive staining around the edges of my preparation (tissue sections, FFPE). I have tried both for antigen...

01 May 2015 1,297 5 View

How can I get rid of these spots on a gDNA Southern?

This is a standard NaOH Southern transfer using good quality agarose. Unincorporated nucleotides were removed, but I still get spots on my film.

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Can I add Pen/strep to my hESC cultures?

Hi, I was just wondering whether I can add antibiotics to avoid contamination into my human embryonic stem cell cultures without interfering with the cells. Any suggestion? concentrations? Thanks...

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How can you "clean" your hESC cultures after splitting them using Colagenase+glass beads?

Hi, I am new in hESC cultures and I was recommended to use colagenase IV and glass beads for the splitting; however, with this system, I always end up transferring my MEF cells into the new flask...

29 October 2014 2,358 5 View