Viacheslav Zemlianski

2 Questions 2 Answers 0 Followers

Questions related from Viacheslav Zemlianski

Can Protein A tag interact directly with secondary antibodies on western blotting?

Hi, everyone! Does anybody have experience with WB of ProtA tagged proteins? Can it give a non-specific signal when you probe the membrane with control ab to different size protein?

30 November 2017 6,082 1 View

Can antibidy react with loading buffer?

Hi, I'm trying to perform some blotting with yeast Trf4 protein, but each time get strange result with nonspecific spots. It appear even in control wells, filled with water+loading dye, but not in...

27 June 2017 3,585 2 View