Veronica Castro-Aceituno

3 Questions 6 Answers 0 Followers

Questions related from Veronica Castro-Aceituno

HMG-CoA reductase activity assay requires the use of any cell line?

Hi, I want to perform the HMG-CoA assay to determinate its activity in the presence of a new extract, I don't know why I think it should be used in some cell line, but so far, any...

07 July 2016 2,486 1 View

How do I stimulate EGF signalin for the evaluation of gene expression in cancer cells?

Hi, I am now testing a possible EGF inhibitor for cancer cells, but after stimulate the cells with external EGF at 20 and 50 ng/ml the RT-PCR bands instead of increase the density is decreasing...

06 June 2015 1,000 8 View

What plasmid DNA do I need for transfection using lipofectamine 2000?

I need to transfect my cells previous performance my experiment. I will use lipofectamine 2000 but in the protocol it said that I need one plasmid DNA, I read about many things related to plasmid...

06 June 2014 3,692 8 View