Verena Zyka

2 Questions 3 Answers 0 Followers

Questions related from Verena Zyka

CRISPRi and CRISPRa - transfection or transduction?

Hi everyone, we recently started using CRISPRi and CRISPRa in our lab and we are struggling to get good results - mainly with CRISRPi. For the gRNAs we used 5 different gRNAs alone or in...

29 June 2020 4,751 3 View

Effect of prolonged transportation of embryonic tissue on neuronal cell culture survival?

Hi everyone, so, we always had good working primary cortical neurons preps (embryonic) and no issue with culturing them to DIV10-14. However, the animal facility now moved to a different location...

01 January 1970 4,960 3 View