Any advice on how to derive clones from hIPSCs, stably transfected with inducible overexpression constructs (problem: spontaneous differentiation)?

I need to derive human IPSC clones from a bulk population that was stably transfected with Dox-inducible (TetOn) overexpression constructs, using the piggybac / transposase system. I did the...

01 November 2017 1,396 2 View