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Questions related from Vera Weisbecker
Does anyone know of a hypothesis as to why there are frogs but no salamanders/newts in Australia? I would like to explain this - if possible - in a lecture but had a look around and can't find any...
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The tissue is fixed in 4% PFA/PBS (no methanol) and ground up for approx. 5 min. in 1% Triton-X 100 and 40 mM Sodium Citrate for subsequent flow cytometry counting of free nuclei - then washed and...
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