Venkatraman Siddharthan

3 Questions 9 Answers 0 Followers

Questions related from Venkatraman Siddharthan

Can anyone know, how to quantify percentage of cells positive from immunofluorescent staining using ImageJ?

For example, immunofluorescent staining of a brain sections with NeuN/NSE (neuronal marker, Alexa 488, green color) and co-staining with calbindin D28K (Alexa 568, red color), I would like to...

02 February 2019 2,548 4 View

Can we perform western blot for EYFP attached with channel rhodopsin2 + AAV from the transducted spinal cord samples?

Hello all, Instead of looking for immunofluorescent of EYFP (as we have some problem), we are planning to do western blot, for EYFP that is attached with human channel rhodopsin 2 and AAV5...

09 September 2014 5,506 1 View

Will expression of the interested protein, behave similar in both stable and transient transfection protocols?

06 June 2013 4,953 12 View