Venkat Ramana

3 Questions 6 Answers 0 Followers

Questions related from Venkat Ramana

Will CIP be active with treatment in tango buffer?

I have a vector with PfoI site where I am planning to insert my gene into that site. However, PfoI is only available at thermo and I use this enzyme over the vector using tango buffer. I might...

12 December 2014 4,753 4 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

12 December 2014 1,905 8 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

07 July 2014 1,414 4 View