Veni Bharti

6 Questions 8 Answers 0 Followers

Questions related from Veni Bharti

Best procedure for extraction of proteins from serum?

Hi, I am looking forward for the extraction method that can extract most of the proteins from the serum for LC-MS analysis. I have many proteins of almost all the molecular weights for full...

02 October 2020 6,797 3 View

Pure antibody in western blot is showing three bands. What does these bands represent?

Hi Everyone, I have tried to run pure antibody (55kD) in 10% SDS/PAGE. I have seen three bands at 250 kD, 75 kD and 50 kD respectively. There is no reducing agent added in antibody. I'm wondering...

20 September 2017 4,568 9 View

How to measure NLRP3 inflammasome activation?

How can I measure NLRP3 inflammasoem activation? In most of the papers, I found protein and mRNA expression levels of NLRP3 and caspase-1. Since NLRP3, Asc and procaspase-1 make an assembly and it...

15 March 2017 4,799 5 View

Maximum concentration of Tween-20 in TBS-T in immunoblot??

Maximum concentration of Tween-20 in TBS-T in immunoblot technique??

15 September 2016 4,944 3 View

Can anyone suggest NAtive PAGE gel conditions?

Please correct me if you have tried any other method or reagents. 1) 12% separating gel (5ml): 30% acrylamide: 4ml, 0.375 M Tris-HCl (pH=8.8): 5.89 ml, 10% APS = 100 ul, TEMED= 10ul 2) Stacking...

08 September 2015 3,574 15 View

Is dopaminergic cell death a consequence of dopamine depletion or vice versa in Parkinson's Disease?

Is dopaminergic cell death is a consequence of dopamine dpletion or vice versa in Parkinson's Disease?

13 August 2015 4,295 5 View