Vasu Saini

2 Questions 12 Answers 0 Followers

Questions related from Vasu Saini

How to conclude microRNA induction leading to upregulation of 3'UTR-reporter luciferase assay ?

Hello everyone, I am experiencing some weird output during my experiments which is as follows : - I am using 3'UTR-reporter luciferase assay to assess the effect of microRNA of my interest but,...

07 November 2017 6,286 1 View

Can someone tell me how much of the cell conditioned medium will be sufficient to isolate 200 ug protein equivalent of exosomes from MDA-MB-231 cell ?

I am using gradient ultracentrifugation procedure in order to do the same. Wherein I am using sorval A-641 rotor at 32900rpm (~120,000g ) for 2 hours for final steps. I am also finding some...

01 January 1970 2,363 2 View