Vasily N Kruzhilin

4 Questions 3 Answers 0 Followers

Questions related from Vasily N Kruzhilin

What are the good substrate materials for Raman microscopy (not SERS)?

Let's suppose we want to have precise measurements of an extremely weak Raman (not enhanced) signal. What substrate materials have the weakest Raman spectra, so will influence the measured spectra...

03 November 2014 5,739 5 View

How can I measure pH in microvolume without a pH meter?

Please could you give me an idea of how to measure pH in a volume ~100 µl if I don't have an appropriate pH meter? I had an idea to use fluorescent ph-sensitive tags.

19 May 2014 1,173 3 View

Could protein disulfide bond conformations differ in X-ray structure (monocrystalline) and lyophilized powder?

I was measuring lyophylized bovine thrombin (purchased form Sigma Aldrich, http://www.sigmaaldrich.com/catalog/product/sigma/t4648 : composition

20 October 2013 2,051 3 View

Evidence of disulfide bridges frequencies in Raman spectra of proteins below 500cm-1

There is theoretical possibility of disulfide bridges confirmation with Raman frequencies from 485cm-1 to 500cm-1, but it is a bit unusual. There are even experimental proofs for some model...

22 March 2013 3,476 3 View