Vartika Mishra

2 Questions 8 Answers 0 Followers

Questions related from Vartika Mishra

Why am I losing dopamine signal in my supernatant samples in HPLC ECD detection?

I am trying to develop a method to detect intracellular and extracellular dopamine in PC12s (undifferentiated). However, I have observed multiple times that I get a signal in my intracellular...

03 March 2014 3,672 17 View

What special conditions should I use for Western blot (e.g. in sample lysate preparation) to probe a membrane protein VMAT2?

VMAT2 is a membrane spanning protein. I use SHSY5Y cells and I tried different methods like RIPA buffer, RIPA with 1% Triton-X and in one instance used syringe to lyse the cells. I do not boil my...

04 April 2013 9,478 4 View