Varshini Pillai

2 Questions 5 Answers 0 Followers

Questions related from Varshini Pillai

How many pairs of primer or single primer does sanger sequencing require?

I am trying to sequence a 3645bp transcript. The coding region is from around 250bp to 1200bps only. So, how many pair or single (either forward or reverse) primers i will be needing for...

17 February 2023 5,316 6 View

Can we use the same primers for qpcr and sanger sequncing?

I will be performing both Sanger sequencing and RT-qpcr for my target gene. For Sanger, I have ordered 3 sets of primers (around 2500, full length). Is it possible to use the same primers for...

16 February 2023 3,635 3 View