Varshiesh Raina

3 Questions 4 Answers 0 Followers

Questions related from Varshiesh Raina

Which is the best transfection reagent to transfect siRNA in SHSY5Y cells?

I have been trying to transfect siRNA in SHSY5Y cells using lipofectamine 2000 but could not find knockdown of my protein of interest.

13 February 2013 3,484 1 View

Is PC12 a reliable in-vitro model to study neuronal differentiation?

I want to study the gene of interest during neuronal differentiation. Although there are many ways of promoting neuronal differentiation; I want to know if NGF-PC12 model system is the best one....

07 November 2012 5,082 5 View

What is the best and easiest way of inducing T Cell activation in-vivo?

I intend to study a protein (X) in context to its role during T Cell activation. It doesn't matter whether it is Th1 or Th2. I need some suggestions like the kind of mitogen, chemically induced etc.

06 November 2012 737 6 View