Varsha P Shetty

2 Questions 3 Answers 0 Followers

Questions related from Varsha P Shetty

How to perform transformation in clinical Klebsiella pneumoniae isolates when they are resistance to antibiotic present in the vector?

I have to perform transformation of a vector into clinical isolates of Klebsiella pneumoniae. My vector consists of chloramphenicol resistance gene but the isolates are resistant to...

21 March 2025 4,264 3 View

How to perform sequential co-transformation in E.coli?

So, initially I have transformed a plasmid into the clinical E.coli isolate. Now, i need to co-tranform these cells with another plasmid of choice. When i tried making the cells with plasmid 1...

21 February 2025 6,433 4 View