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Questions related from Vanessa Sewell
Hello, When I clone multiple fragments into a vector, I get a positive colony PCR. However, when I then purify the plasmid and try to amplify the fragments, they are not coming up in the correct...
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Hello, Could anyone please advise me on the protocol to prepare 1M Creatine Phosphate? Thank you in advance!
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Hello, can anyone please describe to me an effective way to amplify a plasmid backbone. When I use my primers, I get a lot of non-specific priming and do not get a strong enough band (if any) at...
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Hello. I have been doing Maxipreps with plasmids in a pUC19 vector and they worked great! However, when I try to do a plasmid prep for another plasmid, they are failing. I am not getting ANY...
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