Vanessa Maybeck

2 Questions 3 Answers 0 Followers

Questions related from Vanessa Maybeck

Has anyone used the CellAura dyes to label synapses in cortical neurons? Live or fixed?

I'm not interested in kinetics, but I have problems with unspecific binding of antibody labelling in very sparse in vitro cortical neuron cultures. Can the CellAura fluorescent antagonists be used...

12 January 2022 993 0 View

Are there any dyes for live imaging neurons in brightfield (not fluorescent dye)? Is there any way to use Nile Red or XGal?

I need to patch clamp neurons on a substrate that scatters light so I can't use DIC or phase contrast to visualize cells. If I fluorescently label the cells, I have problems with the substrate and...

28 March 2014 3,831 3 View