Vanessa Alizo

2 Questions 4 Answers 0 Followers

Questions related from Vanessa Alizo

Does anyone know the reasons why an unknown gene would show a "bad" amplification curve (no Ct values) during RT-qPCR?

I am trying to do a comparative experiment for differential expression of an unknown gene that is expressed in the brain. I got no Ct values for my unknown but got normal amplification and Ct...

26 November 2020 4,384 1 View

Multiplex Analyses Using Real-Time Quantitative PCR

Hello! I am trying to find more resources on how to perform Multiplex Analyses Using Real-Time Quantitative PCR on brain tissue from punches (.5mm). Because the tissue is so small, the cDNA...

01 January 1970 4,831 3 View