Vanesa Fernández-Moreira

3 Questions 3 Answers 0 Followers

Questions related from Vanesa Fernández-Moreira

Does anyone have a protocol to perfom complex-DNA interaction assay via UV spectroscopy?

I am trying to analyse if my complex interacts with DNA by UV-spectroscopy but I don´t have any experience in these type of experiments, so if someone can send me a reliable protocole it would be...

11 November 2018 2,854 3 View

How to set up a fix method on to the UV-Vis in order to measure the inhibition of the TrxR?

I am trying to assess the inhibition effect of the thioredoxine reductase by UVvis. The problem is that I have to use a fixed wavelength method within the UV-vis spectrophotometer and as I never...

06 June 2018 3,199 2 View

Which UV absorption band do you have to consider when measuring a possible DNA-metal complex interaction by UV-absorption spectroscopy?

The experiment I perform is just subsequent additions of DNA over a solution of the complex. then, I don´t really know if I have to consider the absorption bands of pi-pi* transitions (

03 March 2018 1,799 4 View