10 Questions 13 Answers 0 Followers
Questions related from Vandhana Chez
I managed to isolated polysomes. From the pellet I isolated RNA and proceeded with qPCR. But am surprisingly getting a positive signal for U1snRNA as well.
06 July 2016 5,673 3 View
I want to know the principle behind it
05 July 2016 3,315 6 View
I want to know if I will get a complete 6 kb transcript if I use only one primer specific towards the 3' end of my transcript
26 April 2016 848 6 View
Am working with mammalian cells.Instead of using sucrose gradient I want to use sucrose cushion. Any suggestions?
20 April 2016 9,066 3 View
If so what factors do they need?
09 March 2016 8,945 5 View
I also read that it has Y chromosome. Suggestions from ppl who are working on this cell line is most welcome.
19 February 2016 2,138 2 View
I have all the phyloCSF values for a particular RNA sequence. Need help to make a correlation between the values generated and between the protein/noncoding potential of the sequence.
27 January 2016 6,481 0 View
I can infer difference in proliferation and migration of my cells when subjected to condition media. but when I isolate proteins and run on PAGE gel am not seeing any difference in protein bands....
29 April 2015 1,645 9 View
I store it at 1mg/ml concentration at 4 deg. But when I perform MTT with the same drug after 2 weeks I feel the drug isnt working. I aint sure whether my drug is wrong or is something else going...
19 March 2015 3,756 4 View
I have read protocols which use speed-vac.I dont have access to it.So is there any other technique?
16 March 2015 1,200 5 View