Van Tran

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Questions related from Van Tran

How to denature plasmid from supercoil into relaxed form before cut with restriction enzyme?

I cut 500 ng plasmid in volume 150 ul with 15 ul enzyme Hind III fermentas (exp 2010). Incubate 37oC, 3h after denaturing 37 -> -20 -> 37 -> -20 oC to increase number of linear...

11 November 2016 9,357 8 View

Why does it have smear from the top of gel?

I have to extract DNA from lung ffpe samples with pure link invitrogen kit and high pure PCR template preparation kit Roche. I use 20 ul 20mg/ul RNase A for each sample (about 50 mg tissues).The...

08 August 2016 8,209 5 View