Valerio Piscopo

2 Questions 10 Answers 0 Followers

Questions related from Valerio Piscopo

What are good positive control primers for a NFkB ChIP on 3t3 cells?

Hello everybody, I'm doing a ChIP on an HA-tagged NFkB tranfected in NIH 3T3 cells. For the qPCR I need a gene that is expressed in this cells and whose promoter is bound by NFkB. This will be my...

10 October 2014 6,770 3 View

Cloning mouse TH promoter in a luciferase reporter vector

I have some trouble with this cloning, it seems to be refractory also in a TA vector. Any suggestions? I tried to clone both 8 kb and 4.5kb upstream 5'of the tyrosine hydroxylase gene, first...

05 May 2013 5,318 15 View