Valeriia Kuzyk

5 Questions 9 Answers 0 Followers

Questions related from Valeriia Kuzyk

Wash buffer for reducing non-specific binding in magnetic beads affinity purification?

I'm using VHH nanobodies coupled to NHS-terminated magnetic beads to capture my protein of interest. However the background proteins in my eluate is quite an issue. What can be used as a wash...

06 June 2018 6,932 10 View

Massive protein background with NHS-terminated beads protein purification?

I'm trying to purify a protein from the cell lysate using single-chain antibodies immobilised on NHS-terminated magnetic beads. The yields are pretty good, however I get a lot of background...

04 April 2018 2,530 8 View

Not-freezing conditioned medium?

Hi all! I have recently harvested the conditioned medium from different colorectal cancer cell lines. But some of the flasks wouldn't freeze at -20, and hardly start to get solid at -80. What may...

02 February 2018 1,156 4 View

Optimal protocol for obtaining cell secretome?

I find quite a variety of protocols to obtain cancer cell line secretome (colon adenocarcinoma in particular): starting from 6h in non-FCS medium with 90% confluency, ending with 12h - replace -...

07 July 2017 4,427 2 View

What are the best-recovery methods for protein immunoaffinity purification?

I need to purify serum glycoprotein present in very low concentration (2-10 ng/mL). Current method (sepharose bound monoclonal antibodies with subsequent elution on a filter plate) works, but...

01 January 2017 4,245 4 View