Valeriia Kuzyk

2 Questions 2 Answers 0 Followers

Questions related from Valeriia Kuzyk

Wash buffer for reducing non-specific binding in magnetic beads affinity purification?

I'm using VHH nanobodies coupled to NHS-terminated magnetic beads to capture my protein of interest. However the background proteins in my eluate is quite an issue. What can be used as a wash...

20 June 2018 7,045 9 View

Not-freezing conditioned medium?

Hi all! I have recently harvested the conditioned medium from different colorectal cancer cell lines. But some of the flasks wouldn't freeze at -20, and hardly start to get solid at -80. What may...

01 February 2018 2,895 3 View