Valerie Rose Osterberg

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Questions related from Valerie Rose Osterberg

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

11 February 2021 6,413 3 View

How do I clone Blasticidin or G418 resistance into an expression vector that already expresses my protein of interest?

I would like to conduct a "rescue" experiment in a knock-out cell line that involves designing/cloning a plasmid that expresses my gene of interest and also confers Blasticidin (or G418)...

06 March 2020 1,674 0 View

In IHC, can Thioflavin S and GFP be detected in the same tissue? or will their emissions overlap too much?

I am staining mouse brain tissue that expresses a GFP-tagged protein. I am trying to stain with Thioflavin S. The spectrum for ThS is ex440nm em455-600. The broad emission causes bleed through in...

04 June 2015 5,861 3 View