Valerie Rose Osterberg

4 Questions 12 Answers 0 Followers

Questions related from Valerie Rose Osterberg

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

11 February 2021 6,424 3 View

How do I clone Blasticidin or G418 resistance into an expression vector that already expresses my protein of interest?

I would like to conduct a "rescue" experiment in a knock-out cell line that involves designing/cloning a plasmid that expresses my gene of interest and also confers Blasticidin (or G418)...

06 March 2020 1,676 0 View

Cloning problems: why is my transformation efficiency so low?

I have been trying to clone a 441bp fragment into a 5.6kb AAV vector backbone using the restriction sites AgeI & ApaI. I use SURE2 cells for the transformation to prevent the ITR regions from...

18 September 2015 6,899 14 View

In IHC, can Thioflavin S and GFP be detected in the same tissue? or will their emissions overlap too much?

I am staining mouse brain tissue that expresses a GFP-tagged protein. I am trying to stain with Thioflavin S. The spectrum for ThS is ex440nm em455-600. The broad emission causes bleed through in...

04 June 2015 5,866 3 View