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Our institute is in search of a new Phosphoimager. We currently have a Typhoon that no longer works. Can anyone give me a recommendation on what works well for them, or is the newest technology...
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We have purified our protein of interest, and have shown that FAD is bound, by UV-VIS spectroscopy. We do not understand the role of the FAD binding. I would like to make mutations that disrupt...
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I am purifying a GST-tagged protein, and cleaving the tag with TEV protease. My buffer contains 20 mM HEPES, pH 7.5, 300 mM NaCl, 5 mM DTT. There is 75 mM reduced glutathione around from...
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