Valeria Scagliotti

3 Questions 14 Answers 0 Followers

Questions related from Valeria Scagliotti

Does Cre-driven excision also work on non-integrated plasmid containing loxP sequences?

I have a plasmid to use to generate new transgenic mice, but I would like to try to see my protein expression before starting the transfection of ES cells. The plasmid of interest has the promoter...

10 October 2014 3,643 7 View

How to prepare a 0,2 M phosphate buffer (Na2HPO4-NaH2PO4), pH 6.4?

I'd like to prepare 0.2M solutions of Na2HPO4 and NaH2PO4 and mix the two solutions (I calculated the correct volume of each solution with the Henderson-Hasselback equation) to obtain the right...

05 May 2013 961 37 View

How to prepare the standard curve for Blyscan Glycosaminoglycans assay?

I followed the blyscan protocol using aliquots of 1, 2,3 and 5 ug of standard solution. But at the end of the assay (after using the Dissociation Reagent) when I read the absorbance with a...

05 May 2013 8,557 32 View