I can't transform the recombinant pFB-Lic-Bse into DH10BAC. Do you have any suggestions?

Hi, I'm trying to transform E. coli DH10Bac cells with a pFB-Lic-Bse vector containing my gene of interest. 48 h after transformation, I get some white colony on LB-Km-Tet-Gent-Xgal-IPTG, but when...

01 October 2014 6,666 5 View