4 Questions 8 Answers 0 Followers
Questions related from Uzma Hameed
I want to study the kinetics and thermodynamics properties of cloned, purified alpha amylase. It is active at 90°C. Can anyone suggest a good experimental guide line to set up the experiment to...
06 June 2013 5,855 2 View
I have expressed my protein in E. coli. and I want to purify it. I performed the ammonium sulfate precipitation with 15%, 25%....75% in the indicial samples and ran both the supernatant and...
04 April 2013 6,095 6 View
I have designed 5'phosphorylated linker with sticky end and restriction sites. I have 25 nmol conc of each separate strand. Here is the sample construct: strand 1. 5...
04 April 2013 891 1 View
We have isolated a bacterium from dung for the biogas production. Biochemical tests indicated that it could be Methanosarcina. I want to perform 16s PCR-Ribotyping. Can any one suggest the...
03 March 2012 676 3 View