What are the best medium and freezing components for repeated freezing/thawing cycles of SCS1 E.coli in 384 well plates (storage temp -80°C)?

We have a cDNA expression library cloned into SCS1 E.coli, stored and used in 384 well plate format (as a Mastercopy and several daughter copies). For years, we could generate good copies (in...

13 August 2019 8,206 0 View