What is the minimum amount of rna needed for a one step pcr when using Quantinova SYBR green kit?

I am new to PCR. I am extracting RNA from osteoblast cells and getting very low amount of rna. However i need to do pcr with 12 primers and i will be able to use 20 ng or less per reaction. Is it...

01 January 1970 6,897 4 View