Ursula Chong

3 Questions 2 Answers 0 Followers

Questions related from Ursula Chong

Is puromycin a suitable selection marker for transfection in immortalized keratinocyte (Ker-CT) from ATCC?

Hi, I plan to knock down a gene in immortalized keratinocyte (Ker-CT from ATCC). In the spec sheet, it was mentioned that these cells were immortalized with h-Tert and CDK4 carrying puromycin as...

01 December 2021 9,428 2 View

Do Jurkat E6.1 cells express CD4 surface marker?

I tried staining the Jurkat E6.1 cells with CD4-PE but there were little to no expression. Does Jurkat E6.1 cells express CD4 marker?

27 February 2015 7,897 1 View

Problem with protein separation in 2D gel electrophoresis.

I recently encountered this problem in my 2D gel electrophoresis. The higher molecular weight protein region was not separated perfectly, as the spots appear to be raindrop like or compressed. I...

11 March 2013 5,960 30 View