Uneeb Saliha

2 Questions 1 Answers 0 Followers

Questions related from Uneeb Saliha

Why there is differences in the growth of aroA mutants of two different E.coli strains in minimal media supplemeted with aromatic mix?

I am doing in vitro characterization of two aroA mutants of E.coli using the minimal media (M9) with/without aromatic amino acids to compare the growth of wild-type strains and their mutant. The...

09 November 2021 1,952 0 View

How can i run Gibson construct on agarose gel to see either assembly happens or not? If Some one can share the whole protocol or gel picture?

The size of my vector is 3.8kb and insert is 1.2kb. I have used 70ng of vector and 130ng of insert and mixed with Gibson master mix kept them for 20 minutes at 50C. I want to know is it possible...

20 August 2018 4,679 3 View