Umut Ekin

3 Questions 6 Answers 0 Followers

Questions related from Umut Ekin

Total protein isolation from gram positive bacteria?

I am planning to isolate total proteins of gram positive bacteria. At final stage, I will resuspend the pellet in protein sample buffer that includes 40 mM Tris/HCl (pH 8) , 4 mM EDTA, 8% SDS,...

03 March 2013 7,056 3 View

What is the best procedure to isolate total protein from gram positive bacteria with sonication?

I try to find sufficient parameters such as Hz of sonicator, sonication time, cycles and breaks...etc

03 March 2013 9,199 4 View

Which adjustments of bacterial sonication for total protein isolation are reliable?

Taking samples during sonication and: 1)Visualize under microscope to see burst cells 2)Measure Absorbance at 280 for DNA/RNA release 3)Centrifuge and measure the protein concentration by...

03 March 2013 9,405 4 View