Ummay Honi

4 Questions 5 Answers 0 Followers

Questions related from Ummay Honi

Need suggestions for getting better result in LR reaction during Gene transfer through Gateway technoloy?

I want to transfer a gene. I have cloned the gene in TOPO TA cloning vector from invitrogen. Now I am doing LR reaction with LR Clonase™ II Plus enzyme from invitrogen. My insert size is 1374bp...

19 February 2018 2,058 4 View

How to convert fmol to nanogram during using LR clonase ii plus enzyme in LR reaction?

According to manual the formula is ng = ( x fmol )( N )(660 fg/fmol)(1 ng/106fg) where N is the size of the DNA in base pairs, and x is the number of fmoles. so , if my entry clone is 2817bp and...

17 February 2018 9,843 3 View

Can any one please give me suggestion , why i am getting sabnormal expression change in q-RT pcr?

I have sprayed my 30d old plants with Gibberelllic acid (GA3)(100ppm) and collected sample for RNA isolation after 2h,4h,8h,12h and 24h of spray. After that I did q-Rt for GA biosynthetic genes...

15 February 2017 3,774 5 View

Can any one please give me suggestion , why i am getting such a abnormal expression change in q-RT PCR?

I have sprayed my 30d old plants with Gibberelllic acid (GA3) and collected sample for RNA isolation after 2h,4h,8h,12h and 24h of spray. After that I did q-Rt for GA biosynthetic genes with this...

15 February 2017 1,509 9 View