Ummay Ayman

3 Questions 6 Answers 0 Followers

Questions related from Ummay Ayman

Transfection of neuroblastoma cell line (SHSY5Y) for RNA collection??

Usually neuroblastoma have low transfection efficacy (5-10%) when transfected with Lipofectamine 3000. So, this time I have tried electroporation with this condition, poring pulse: 175V, 2.5ms,...

24 August 2023 1,964 2 View

Seeding density of SH-SY5Y cell line in 8 well plate?

Hello everyone, would you please suggest me the optimum seeding density of SH-SY5Y in 8 well plate to do transfection using lipofectamine 3000? Thanks in advance. Best Ayman

01 March 2023 1,660 3 View

What are the reasons of getting insert band at wrong size after RE digestion?

I have tagged pEGFP_N1 with APOE insert. However, after RE digestion, at gel electrophoresis, I am getting the insert band at wrong size. I have used EcoRI and SalI restriction enzymes. What could...

22 February 2023 4,764 8 View