Udaykumar Kage

4 Questions 5 Answers 0 Followers

Questions related from Udaykumar Kage

Can anyone tell how to start a conversation for collaboration with an unknown researcher?

I just wanted to collaborate with another lab who are already well experienced with the project I wanted to start in plants but they work in mammals. This collaboration is not to write an new...

06 June 2018 3,440 4 View

How to get rid of bacterial and fungal contamination during callus induction from wheat embryos??

We are inducing callus from wheat embryos (media-MS+2,4-D), getting lot of contamination (both bacterial and fungal). Which is seems to be arising from seeds not the media, as its appearing around...

06 June 2016 3,112 4 View

After protein expression, can the cell suspension stored at 4 degree for 2 days be used for protein purification??

Hello everyone, I have expressed my protein in e.coli (BL21) cells at 19 degrees for 14 hours and stored the culture at 4 degrees for 2 days without pelleting it out. After running SDS PAGE, i...

03 March 2015 4,477 8 View

Gene overexpression in arabidopsis to elucidate gene function. Only coding or with UTR region?

Hello everyone, I am intended to over express one gene in arabidopsis. I just wondering to know whether cloning gene (with UTR regions without introns) into vector through TA cloning and...

01 January 2015 9,231 9 View