Uchechukwu Chikezie

3 Questions 7 Answers 0 Followers

Questions related from Uchechukwu Chikezie

How do I design and clone two different DNA / gene constructs into a cloning vector for protein expression ?

Hi. all. I want to express two different target proteins in. E.coli. How do I clone the two different DNA/gene constructs into a cloning vector, to enable expression of the two target...

10 September 2019 1,603 4 View

Please i need help with explaining my '' messy '' sequencing result ?

Hi. i did a cloning reaction [ my insert is the gene for the PH domain ] using the Gibson Assembly cloning technique and used the products of the cloning reaction to...

08 September 2018 8,545 12 View

Structure-based Multiple Sequence Alignment Tools and Tools for predicting the domains on a query protein sequence.

Hi all, I am working on the PH domain of a mouse protein and i want to run a structure-based multiple sequence alignment to try to predict the boundaries of the PH domain and the predicted...

01 January 1970 5,894 5 View