Tzu Shan Ng

2 Questions 5 Answers 0 Followers

Questions related from Tzu Shan Ng

Checking RNA integrity through TAE gel?

I have tried to check my RNA integrity through TAE gel. I managed to obtain two bands here, but there are some "clots" in the well which are stained by EtBr. I am not sure what this is, therefore...

04 April 2013 4,678 34 View

Standardization of cDNA or RNA for qPCR

I am confused if I should standardize the amount of RNA or cDNA for the qPCR run. If lets say we have standardized the amount of RNA for RT-PCR, but we cannot guarantee the efficiency of RT-PCR...

04 April 2013 4,979 6 View