Tzong-Han Lin

5 Questions 3 Answers 0 Followers

Questions related from Tzong-Han Lin

How to download fasta files in "gene features" format from NCBI with multiple samples in R?

Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...

05 July 2024 10,106 1 View

Impact of nuclear-mitochondrial DNA segments (NUMTs) in phylogeny construction?

"Nuclear-mitochondrial DNA segments (NUMTs) are mitochondrial DNA (mtDNA) fragments that have been inserted into the nuclear genome." (Xue et al., 2023) I would like to know under this...

21 June 2024 754 1 View

Could specimen preserved in bad condition get polluted by other sample in the same container?

I got some lizard specimen which had been stored in 95% alcohol for over 15 years at room temperature. Also, the lizards were all being preserved in the same container, while their legs were...

21 July 2022 676 2 View

Is it OK to analyze PCR products with different cycles (with same primers) ?

Hello~ I had some issues with PCR during recent experiments, while some of the sequences having high quality scores, the others are pretty bad. If I re-do PCR on those products, would it be OK to...

03 March 2022 8,708 3 View

The phylogenetic backbone for testing gene flow?

Hi everyone. I'm currently working on a project related to mito-nuclear discordance of our focal taxa, in which the phylogeny constructed by mitochondrial sequences differed from SNP data. My...

01 January 1970 8,660 2 View