Tyler T Cooper

2 Questions 5 Answers 0 Followers

Questions related from Tyler T Cooper

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

10 October 2017 1,100 2 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

07 July 2014 740 1 View